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Genesis o f our Success Story Early in 1991, the vision of two instrumentation professionals, to offer the agricultural and biological researchers, products and solution totally indigenous in par with imported alternatives gave birth to PELICAN EQUIPMENTS. It was a humble beginning with crude and raw technical ideas, a playful passing thought of two professionals to begin products for Protein, Fat and Fiber estimation to challenge the foreign principals.
OUR THREE-TIER MANTRA:
Professionalism has been the foundation of our success. It has been our way of thinking and life - Applying Scientific knowledge to our work.
Trust is something we earned over time, not through our words but through our actions - as a Trust Worthy & Customer Oriented Organization.
Our Motto: In Pursuit of Perfection - In all that we do”. Perfection is our Goal. Excellence can be tolerated.
(Together Everyone Achieves Maximum)
NSIC (GOVT. OF INDIA) REGISTERED ORGANISATION
We would like to highlight that we have been permanently enlisted with National Small Industries Corporation (Government of India Organization) under single point registration scheme in par with DGS&D. Our NSIC Regn. No. NSIC (C) GP/17 (5336)/2003 dt 06.11.2003.
KEL PLUS AUTOMATIC NITROGEN / PROTEIN ESTIMATION SYSTEM
Fertilizers which have Ammonia and Urea as key components are found to contain Nitrogen. Nitrogen components like TNT, Nitrocellulose etc. are key compounds in Explosives. Heterocyclic Nitrogen Compounds are important in production of Plastic, Rubber and Paint. The purity of water is evaluated in knowing the Nitrogen content of water. Nitrogen has proved to be an excellent indicator. For example the BTU potential of a sample of coal can be calculated based on the N2 content.
Nitrogen determination has a long history in the area of analytical Chemistry. Johan Kjeldahl first introduced Kjeldahl Nitrogen method in 1883 at meeting of the Danish Chemical Society. As a Chairman of Chemistry department of the Carlsberg laboratorium near Copenhagen, Kjeldahl was assigned, to scientifically observe the processes involved in beer making. While studying proteins during production he developed a method of determining Nitrogen content that was faster and more accurate than any method available at that time.
Representative sample is essential if analytical data are to be meaningful. The samples must be also homogeneous. The necessity for consideration of homogeneity increases as sample size for analytical procedure decreases. To assure homogeneity samples are usually dried and ground prior to analysis. In case of liquid samples, samples are collected in new or acid washed glass polythene bottles. They should be analysed as quickly as possible. If immediate analysis is not possible samples can be preserved for Kjeldhal digestion by acidifying to PH 1.5 to 2.0 with concentrated sulphuric acid and storing at 4°C. For analysis of Nutrients in plant materials previous wet digestion of sample is recommended. Rapid changes in the nitrate and ammonia contents of soil samples occur after removal of the samples from field or pot, because of the increased aeration and rise in temperature. It is, therefore, desirable that the extraction of nitrates and ammonium follow the collection of the samples closely.
Very early Kjeldhal digestion and distillation were performed using stone fume hoods and gas mantles as a heat source. In 1920’s this was replaced by classical Macro Kjeldhal digestion and distillation apparatus using Kjeldhal flask from 500-800ml volume.Latter variation in equipment made use of Aluminium heating blocks designed to accept number of straight digestion tubes at once simultaneously. The latest advancement in block digestion techniques by Kjeldhal procedure gave birth to automatic Nitrogen and protein analyzer in the world market from countries like Sweden, Germany, Japan, etc which made pioneering efforts for automation.
Like any other method, the Kjeldhal procedure is subject to problems with methodology, technique and equipment. Understanding the principles behind the Kjeldahl process and using appropriate quality control procedures should prevent most problems from occurring. When developing a new or modifying an existing method, one should proceed which caution and experimental verification.
Oils and Fats are the triglycerides of fatty acids in which one molecule of glycerol has combined with three molecules of long chain fatty acids with the Elimination of water. In addition to triglycerides, the oils and fats may contain a total of 2% free fatty acids, and sterols, phospholipids coloring material and hydrocarbons.
Chemistry Of Crude Fibre
Crude fibre refers to the residue of a feed that is insoluble after successive,boiling with dilute acid and alkali. This method was originally proposed at the weende experiment station. Hence this method on determination of crude fibre is known as Weende s method of determination of crude fibre.
Crude fibre is the portion of the total carbohydrate of a food that is resistant to the acid and alkali treatment.
The Weende scheme of proximate analysis is to separate the total carbohydrate into two categories viz. crude fibre and Nitrogen free extractives. Nitrogen free extractives represents the soluble sugar starches,glycogen,some fraction of hemicellulose and of course other water soluble components like water soluble vitamins etc.
As per Weende method, crude fibre is the fraction of carbohydrate that remains after treatment with acid and alkali.
Effect of acid and alkali treatment on a fat free food
But this method any insoluble material after the acid and alkali digestion will be made up of (1)all original cellulose (2) variable proportion of hemicellulose (3) a small but highly variable portion of lignin.
In the Weendes method when the sample is subjected to acid and alkali digestion, we obtain a residue comprising ash (mineral matter) of the feed and the resistant fraction of carbohydrate. When the residue is ignited the organic matter gets oxidized leaving the inorganic residue or ash. Thus the difference in weight of the residue before and after ashing gives the weight of crude fiber.
S. S. Samuel (Managing Director)
No. 38, 2nd Floor, Burkit Road, T. Nagar
Chennai - 600017, Tamil Nadu, India